Oxford Nanopore MinION Workshop


Day 1 - Monday 25 March

Venue: UQ Centre for Clinical Research, The Royal Brisbane & Women’s Hospital Campus

Oxford Nanopore experts, Alina Ham PhD, (Technical Applications Specialist) and Androo Markham PhD, (Field Applications Specialist) will provide attendees with a nanopore sequencing overview and case study discussion.

A nanopore sequencing demonstration as well as a rapid sequencing control experiment will allow each participant to gain practical hands-on experience of running the MinION/GridION.

The workshop will cover the following:

  • Comprehensive technology overview

  • Library preparation in groups

  • Library loading practice

  • Running the MinION/GridION

  • Introduction to basecalling and EPI2ME analysis workflow


Day 2 - Tuesday 26 March

Venue: Clinical Skills Development Service, Royal Brisbane & Women’s Hospital Campus

Oxford Nanopore experts will provide attendees with a comprehensive data analysis overview followed by practical hands-on experience running different analysis workflows.

This workshop will give participants experience with real-time analysis tools as well as in-depth analysis workflows with command line tools.

The workshop will cover the following:

  • In depth overview of EPI2ME for real-time end-to-end analysis

  • Command line tutorial

  • Guppy (command, parameters and folder structure)

  • Overview of fast5 file format

  • Quality and read length distribution plot and filtering

  • Mapping to a reference

  • De novo assembly and consensus correction